Purification and Properties of Apple Fruit Malic Enzymel

Summarwy. \ialic enzyme wxas iso)latedl and plurifie(l froimi mature apple fruits (ainus svivcstris, Miller ) by utilizing proce(lures prol)al)ly applicable to other soluble enzymes in this and similahr tissues. The physical properties of apple fruit iinalic enzymle are similar to those reported for malic enzyme fromn other plant and( animilal sources. It is specific for L-malate, TPN and requires a divalent cationi for activity. In conitrast to the pigeon liver enzyme. supplemental TPN is not required for oxalacetic decarboxylase activity of the fruit enzvymie. The pH opltimum of the malic enzymne varied widh1 the i-.malalte conicenitrationl and the nature of the divalent catioll presellt. n-Alalate a.ctivate(l the oxi(latioil of L-malate at rate-liniting colucenitratioiis.